TABLE 2.

Percentage of env Sequences extended to gaga

Resistance mutation(s)% env sequences extended to gag
5 h after infection9 h after infection
UnpretreatedHydroxyurea pretreatedUnpretreatedHydroxyurea pretreated
None (pNL4-3)71 ± 1871 ± 1586 ± 186 ± 4
M41L + T215Y74 ± 1146 ± 10**76 ± 784 ± 9
M184V77 ± 954 ± 14*81 ± 1581 ± 11
BV34 mutationsb35 ± 7**3 ± 4**86 ± 266 ± 50
  • a Unpretreated P4 cells or cells cultured in the presence of 250 μM hydroxyurea for 18 h were infected with virions expressing the indicated resistance mutations. The numbers of transcripts extended to env and gag were quantified by real-time PCR. Results are expressed as follows: (number of gag sequences)/(number of env sequences) × 100. Results are the means ± SD for 8 to 10 experiments (5 h) or 2 experiments (9 h). *, P < 0.05 compared to WT virus; **, P < 0.01 compared to WT virus.

  • b Seven resistance mutations carried by clinical isolate BV34 (see Results).