TABLE 2.

Mutations and titers of JFH-1 replicon in 293 clones

CloneNucleotideaAmino acidbRegionReplicon titer (copies/μg of RNA)
1None5.11 × 106
2None3.47 × 106
3None6.82 × 106
4None8.33 × 106
5None5.93 × 106
65897T→C2404 L→PNS5a5.57 × 106
6420A→CSynonymouscNS5b
7None3.65 × 106
83195T→CSynonymousNS35.10 × 106
9None3.73 × 106
  • a Position of mutated nucleotide within subgenomic replicon.

  • b Position of mutated amino acid within complete ORF of full-length JFH-1.

  • c Synonymous mutation results in no change to amino acid sequence.