TABLE 1.

Mutations and titers of JFH-1 replicon in HeLa clones

CloneNucleotideaAmino acidbRegionReplicon titer (copies/μg of RNA)
1None6.43 × 106
25500T→C2272S→PNS5a5.69 × 106
7182T→CSynonymouscNS5b
7217A→G2844H→RNS5b
37820A→GNAd3′-UTR3.05 × 106
45681C→A2332T→KNS5a1.07 × 107
6672T→CSynonymousNS5b
53643A→G1653M→VNS32.56 × 106
5851G→A2389A→TNS5a
5914G→A2410E→KNS5a
62474C→G1263A→GNS34.38 × 106
72454A→GSynonymousNS34.66 × 106
8361T→CSynonymousCoree8.31 × 105
5673A→GSynonymousNS5a
6648G→ASynonymousNS5b
97097T→C2804L→PNS5b6.81 × 106
  • a Position of mutated nucleotide within subgenomic replicon.

  • b Position of mutated amino acid within complete ORF of full-length JFH-1.

  • c Synonymous mutation does not change amino acid sequence.

  • d NA, not applicable.

  • e Sequential region from 5′-UTR upstream of neor gene.