TABLE 1.

Efficiency of cosmid-based construction of recombinant OAdV genomes

Cloning vectorInsertE. coli strainCloning efficiency (no. of colonies per μg of DNA)No. of clones positive in PCR/totalInsert orientation (no. positive/no. tested)
o1o2Ma
pOAdV1-cosRSV-haatDH5α7.6 × 1034/62/42/4-
JM1092.4 × 1046/61/43/4-
pOAdV2-cosRSV-haatDH5α3.0 × 1044/6NDNDND
JM1092.8 × 1045/62/42/4-
pOAdV3-cosRSV-haatDH5α1.2 × 1048/84/81/83/8
JM1097.5 × 1038/82/82/84/8
  • a M, multiple insertions; ND, not determined.