TABLE 1.

Primer pairs used to differentiate between the ΔG/HEK and ΔNS1/2 viruses based on product size and the presence or absence of HEK point mutations in the F gene

Forward primeraReverse primeraVirusProduct sizeb (bp)
ABwt rA21,327
ΔG/HEK1,327
ΔNS1/2273
CDwt rA21,801
ΔG/HEK852
ΔNS1/21,801
CE-wtwt rA21,954
ΔG/HEKNone
ΔNS1/21,954
CE-HEKwt rA2/HEK1,963
ΔG/HEK1,014
ΔNS1/2None
1406514535All above521
  • a See Materials and Methods for exact sequence positions and Fig. 1 for general location in the RSV gene map.

  • b Primer pairs A-B and C-D differentiate between ΔG/HEK and ΔNS1/2 based on product size. Primer pairs C-E-wt and C-E-HEK prime only on wt F sequence (found in ΔNS1/2) and HEK F sequence (found in ΔG/HEK), respectively. Primer pair 14065-14535 primes on both wt L sequence (found in ΔNS1/2) and SITES L sequence (found in ΔG/HEK), which are then differentiated by nucleotide sequencing.