TABLE 1.

Characteristics of serum samples from healthy blood donors and from patients before or after administration of Addl1520a

Sample source and no.Time after Ad administrationAnti-Ad titerbNA titercReactivity againstd:
HexonPbFiber
DNDNDN
Patient
    1D0ND256++++++++/−++
D10ND512++++++++/−++
    2D0ND2,048+++++++
D10ND4,096++++++++
    3D0256256+/−+++
D108,1922,048+/−+++++++++++/−
    4D05121,024++
D108,1928,192+/−+++++++++++++
    5D0256256++++
D108,1924,096++++++++++
    6D01,024128+++
D104,096512++++++
    7D05128+++
D1016,384512+++++
    8D01288+++
D108,1924,096++++++++++++
    9D0ND256+++++++++++
D10ND512++++++++++++++
    10D0ND8++++
D10ND8++++
    11D0ND64+++++++++++
D10ND128+++++++++++
    12D0ND128+++++++++++++
D10ND256+++++++++++++
    13D0ND32+++
D10ND32+++
    14D0ND256++++++++++++++
D10ND256++++++++++++++++
    15D0ND128+++
D10ND128+++
    16D0ND2,048++++++++
D10ND4,096+++++++++
    17D0ND16++
D10ND16++
Healthy blood donors
    18D0ND>1,024+/−++++++++++++
    19D0ND>1,024++++++++
    20D0ND>1,024++++++++++++++++
    21D0ND>1,024++++++++++++
  • a Serum samples were taken before (D0) or 10 days after (D10) Ad administration. ND, not determined.

  • b Sera were assayed for anti-Ad antibody by enzyme-linked immunosorbent assay, and the titers are expressed as the reciprocal of the serum dilution.

  • c The NA titer is expressed as the reciprocal of the serum dilution and was indirectly determined by the inhibition of green fluorescent protein expression in HeLa cells infected with Ad5GFP vector.

  • d The reactivity against the native (N) or SDS-denatured (D) Ad capsid proteins hexon, Pb, and fiber was evaluated by Western blot analysis.