TABLE 2.

Phagotopes isolated by phage biopanning of Pb antibodiesa

ClassSequencePatient no.
I (epitopes identified only in D0 51GRNSIRYSEL60
samples [preexisting Ab]) NVRYSW 1
GRYAES 1
RVRYFI 1
216RLGFDPVTGLV226
LGLDPG 2
AVTGID 2
336DHAIRGDTFA345
RGDVTF 1
II (epitopes identified only in 69VYLVDNKST79
D10 samples [newly acquired SLVDNS 3
Ab]) RNLFVD 4
YMSVNV 3
193 LKVGRQNG200
KGQRFR 3
VNKGQR 3
LMRVGS 3
PHVRGS 4
223TGLVMPGV230
VMLPWL 4
VMLPRR 3
SHPGAV 3
551CPYVYKALGI560
KVYKYF 4
PYVIWK 3
EGIYKT 3
III (epitopes common to D0 and 110TINLDDRSHWG120
D10 samples [preexisting and FIYLDD 3
newly acquired Ab]) LDD-S-YS 2
ASFDRS 4
YTLLDR 4
LDD----WGV 3
DRRWHR 4
MSD--HWR 4
NRSMWQ 1
NRSMWQ 1
311NNSGSGAEEN320
YMSGSG 3
YMSGSG 3
HSGAEF 2
398STFTQYRSWYL408
NFTQVG 2
NFTQVG 2
TAGGQY 1
TAGGQY 1
QTNYRG 3
GQYSSS 2
RYGWYL 3
  • a The phagotopes recovered from immobilized Pb-specific Pb antibodies (Ab) were grouped according to conserved residues and peptide motifs and then aligned with homologous regions of the Ad Pb sequence (underlined, with residue numbers as indicated). Homologous or identical residues at similar locations are in boldface. Patient numbers correspond to the patient identification numbers in Table 1. Note that motif LDD-WGV likely corresponded to a discontinuous epitope.