TABLE 1.

Defining regions of PAT1 and Us11 required for interaction in the yeast two-hybrid system

PAT1 proteinInteractiona of:
WT Us11Us11Δ1-87Us11Δ88-155
WT++
Δ281ND
Δ352ND
Δ412
Δ436ND
Δ1-40++
  • a PAT1 proteins were expressed in yeast cells as activation domain fusions. Us11 derivatives were expressed in yeast fused to the LexA DNA-binding domain. Interaction between Us11 and PAT1 was detected by the ability to form blue colonies on indicator plates (+). −, white colonies; ND, not determined.