TABLE 1.

Colocalization of CypA with viral factories in cells infected with VV mutant ts53a

Virus (temp °C)12 h p.i.Shiftdown to 31°C
2 h4 h
No. of cellsColocalization (%)No. of cellsColocalization (%)No. of cellsColocalization (%)
WT (31)396349 (88.1)
WT (40)224165 (73.7)
ts53 (31)234176 (75.2)
ts53 (40)37372 (19.3)249120 (48.2)305242 (79.3)
  • a BSC-40 cells were infected with WT VV or ts53 (MOI = 5) at both 31 and 40°C and harvested at 12 h p.i. for immunofluorescence microscopy. A sample of cells infected with ts53 at 40°C for 12 h was shifted to 31°C for an additional 2 or 4 h. The cells in more than 40 random fields were counted.