TABLE 1.

PCR, overlap extension PCR, and mutagenic primersa

ConstructPrimerSequence
Ad37s/Ad5kL37TGT CTT GAA TCC AAG ATG∗ AAG CGC GCC CGC CCC AGC GAA GAT GAC TTC
37s5k-3TGG AGC TGG TGT GGT CCA CAA AGT GCG CGT GTC ATA TTC TGG GTT CCA
5k-5ACT TTG TGG ACC ACA CCA GCT CCA
fiber3CAT AAC GCG GCC GCT TCT TTA TTC TTG GGC
Ad5s/Ad37kfiber5ATG GGA TCC AAG ATG∗ AAG CGC GCA AGA CCG
5s-3TGG TGT GGT CCA CAA AGT TAG CTT ATC ATT
5s37k-5AAG CTA ACT TTG TGG ACC ACA CCA GAC ACA TCT CCA AAC TGC ACA ATT
37frAAA CAC GGC GGC CGC TCT TTC ATT CTT G
Ad5Δsshort3GCT TAG GTT AAC CTC AAG CTT TTT CTT GGT TTT TTT GAG AGG TGG GCT
short5AGC CCA CCT CTC AAA AAA ACC AGG AAA AAG CTT GAG GTT AAC CTA AGC
Ad5s/Ad37srep3-3ATC AGT ATT AAC TTG CAG TGG AGC CTT AGG GTT TAC AGT TAG GCT TCC GGC CTC GTC CAG AGA GAG GCC GTT
rep3-5GGA AGC CTA ACT GTA AAC CCT AAG GCT CCA CTG CAA GTT AAT ACT GAT TCA AAC ATA AAC CTG GAA ATA TCT
rep7-3ATC ATT GTC AAA TGT CAA CCC TTC TCT TGC TCT TAC ATT TAT ACC AAT GTT GTA ATC AAA TTC TAG GCC ATG
rep7-5ATT GGT ATA AAT GTA AGA GCA AGA GAA GGG TTG ACA TTT GAC AAT GAT GGT GCC ATT ACA GTA GGA AAC AAA
  • a Introduced restriction sites are in bold. The start codons are denoted by an asterisk. Mutagenic bases are underlined.