TABLE 1.

Oligonucleotides used for mutagenesis

Oligo-nucleotideaSequenceb
T5P (S)5′-ATGGACATTGACCCCTATAAAGAATTTGGA-3′
T5P (AS)5′-TCCAAATTCTTTATAGGGGTCAATGTCCAT-3′
L97I (S)5′-ATGGGCCTAAAAATCAGACAACTACTGTGG-3′
L97I (AS)5′-CCACAGTAGTTGTCTGATTTTTAGGCCCAT-3′
P5T (S)5′-ATGGACATTGACACGTATAAAGAATTTGGA-3′
P5T (AS)5′-TCCAAATTCTTTATACGTGTCAATGTCCAT-3′
  • a S, sense polarity; AS, antisense polarity.

  • b Mutated codons are underlined, mutation sites are in boldface.