Effect of Y802W803 substitution on the fusogenic capacity of HIV-1 Env glycoprotein

MutantNo. of syncytia/well (293T/HeLa P4 cocultivation)a% of wtbPc
NTd    0NDeND
pcEnv-wt719 ± 49100ND
pcEnv-Y802W803-SL546 ± 8876<0.02
pcEnv-Y712-A1,149 ± 74159<0.001
  • a Values shown are the numbers of syncytia obtained per well and are the averages of three independent experiments performed in triplicate.

  • b Data are numbers of syncytia divided by the number obtained with wt pEnv-wt (719 ± 49 syncytia/well).

  • c The results for the mutant Env glycoproteins were compared to those for the wt by means of the t test for unpaired samples.

  • d NT, cells transfected with the pcDNA3 vector alone.

  • e ND, not determined.