TABLE 3.

Prion incubation time in mice following high-dose oral inoculationa

GenotypeME7.1RML 5.0
No. of mice with disease/no. in groupMean incubation time (days) ± SDNo. of mice with disease/no. in groupMean incubation time (days) ± SD
IL-10−/−5/585 ± 105/581 ± 1
129Sv5/5271 ± 35/5217 ± 3
  • a IL-10−/− and 129Sv mice were inoculated by the oral route with a 200-μl volume of a 10−1 dilution of 10% (wt/vol) ME7.1 diluted in PBS or RML 5.0 diluted in PBS plus 5% BSA. Inoculated mice were monitored daily for clinical signs of mouse prion disease. Mice that succumbed to terminal prion disease were euthanized at the point of neurological disease and dysfunction.