TABLE 1.

Incubation time for prion disease in various mouse strains inoculated with RML 5.0 by different routesa

Inoculation routeGenotype10−1 inoculum10−4 inoculum
No. of mice with disease (n = 5)Mean incubation time (days) ± SDNo. of mice with disease (n = 5)Mean incubation time (days) ± SD
IntracerebralIL-10−/−558 ± 14***5112 ± 7***
129Sv5140 ± 25153 ± 4
IL-4−/−5137 ± 235166 ± 2**
IL-13−/−5141 ± 135156 ± 14**
IL-4/IL-13−/−5148 ± 35180 ± 5**
BALB/c5145 ± 15195 ± 0
PeripheralIL-10−/−5119 ± 0***5138 ± 0***
129Sv5186 ± 05200 ± 2
IL-4−/−5165 ± 295183 ± 34
IL-13−/−5162 ± 0**5204 ± 0
IL-4/IL-13−/−5173 ± 0*5219 ± 4
BALB/c5203 ± 155220 ± 37
  • a IL-4−/−, IL-10−/−, IL-13−/−, IL-4/IL-13−/−, and control BALB/c and 129Sv mice (n = 5 per group) were inoculated with a 10−1 or 10−4 dilution of 10% (wt/vol) RML 5.0. Inoculated mice were monitored daily for clinical signs of mouse prion disease. Mice that succumbed to terminal prion disease were euthanized at the point of neurological disease and dysfunction. Statistical analysis was carried out with the paired samples t test. *, P ≤ 0.01 (comparison with BALB/c mice); **, P ≤ 0.003 (comparison with BALB/c mice); ***, P ≤ 0.001 (comparison with 129Sv mice).