TABLE 1.

Search primers used for identification of transposon insertions into pSM3fr

PrimerSequence
M23-for5′-TCT TCA GCG ACT TTG CCA C-3′
M23-rev5′-CGA GCG GAT CGA TGA TGA AG-3′
M24-for5′-GAG AAA GGA GGA AAA GTG GG-3′
M24-rev5′-GAT CGA CGG TTC ATC GTT CG-3′
m25-for5′-GTA ATT CTT GGG CCA GGT CAG C-3′
m25-1-rev5′-GCA TTG TTC TCG TCA ATC CG-3′
m25-2-rev5′-ACA ACC TCA CGC AGC TCA TCT C-3′
M36-for5′-CTC CCG AGG AAG AGA TTG TG-3′
M36-rev5′-CTG CTT GCG TCA AAA TGC TC-3′
M43-for5′-CTC GTC CGT GCA GAG AAT CAT C-3′
M43-rev5′-CTG ACA GCA TCG AGA ATC C-3′
m128-for5′-GCT CGC TCG ATC CAT TCT TC-3′
m128-rev5′-GGC GTG AAC ACG ATG TTC TTG-3′
m139-for5′-CAG ATA CTT GGA CAG GTC CAC G-3′
m139-rev5′-CTT GGA GAG GCA AGA GAG TCA C-3′
m140-for5′-CAG GTG GTG GAT GCT GAA G-3′
m140-rev5′-CTC CAC CTA GCT CAC TAA CAG C-3′
m141-for5′-CTC GAA GCT CGA AGA GAA CAC C-3′
m141-rev5′-CAT CAC ATA AAC CCC TCC CC-3′
m142-for5′-ACA CAC ACA CCA ACC ACC CTT C-3′
m142-rev5′-GGC GGG TGA CAT GAG AGA T-3′
m143-for5′-CGA TAG ATA GAA GAT GCT GCC C-3′
m143-rev5′-CAC CCG GAT GGT GTA AAA GAG-3′
m152-for5′-TCC ACC TTC AAT CGT CCA CC-3′
m152-rev5′-GGA TAC GGA GGA GAA TGT GGT G-3′