TABLE 2.

In vitro growth differences between wild-type MCMV and the US22 gene mutants at day 6 postinfection in fibroblasts, macrophages, and endothelial cells

MCMV mutantIn vitro growtha
NIH 3T3 fibroblastsIC-21 macrophagesJ774-A1 macrophagesPECSVEC4-10
Tn-M36+++++/+++++
Tn-M43++b++++
Tn-m139+++
Tn-m140−/+++/+++−/+
Tn-m141−/+++/+++
  • a −, no difference; +, ++, and +++, 1, 2, and 3 log units of difference, respectively; −/+, <1 log unit of difference.

  • b The titers were at least 2 log units lower when titrated on NIH 3T3 cells.