TABLE 1.

Phenotypes of recombinant RNPs with point mutations in PB2a

VirusProtein expressioncRNAvRNAmRNAIn vitro transcriptionEndonuclease activity
Wild type++++++++++++++++++++++++
T24G++++++++++++++++++NDND
W49A+++++++++++++++
W49Y+++++++++++++NDND
W78A+/−NDNDNDNDND
W78Y+++NDNDNDNDND
D83T++++++++++++++++++NDND
W99A++++NDNDNDNDND
W99Y++++NDNDNDNDND
F130A++++NANA
F130Y++++NDNDNDNDND
R142A+++++++++++++++
R142K+++++++++++++NDND
K190A++++++++++++++++NDND
K190R++++NDNDNDNDND
  • a ND, not determined; ++++, 100% of wild-type value; −, no activity. NA, not applicable.