TABLE 1.

Oligonucleotides used for mutagenesis and cloning (involving PCR amplification)

OligonucleotideSequence (5′-3′)
PVcre (SacI) forwardGCC GAG CTC ACA ACC TAT GGG TTC GGA ACT ATT AAC AAC TAC ATA C
PVcre (SacI) reverseGC GAG CTC TAC TAG CAA ACA TAC TGG
HRV14cre (SacI) forwardGCC GAG CTC ACA ACC TAT GGG TTC GGA GCA CTC ACT GAA GGC TTA G
HRV14cre (SacI) reverseGGC GAG CTC TTT TTG TGT GTG TTT TGG
HRV2cre (SacI) forwardGCC GAG CTC ACA ACC TAT GGG TTC GGA GAT TTA ATC ATT TAC CGA AC
HRV2cre (SacI) reverseGGC GAG CTC GGG AAT GTA ATC ATC ACC
HRV2cre U11C forw. (QM)aATT TAC CGA ACA AAC ACC GTA GGT GAT GAT TAC
PVcre (EcoRI) forwardCGG AAT TCA CTA TTA ACA ACT ACA TAC AG
PVcre (EcoRI) reverseCGG AAT TCC CCA AAG CCG TAA GTC GTT ACT AGC AAA CAT ACT GG
PVcre G3A C11U forw. (QM)CAT ACA GTT CAA GAA CAA ACA CTG TAT TGA ACC AGT ATG
PVcre A14C forward (QM)CAA GAG CAA ACA CCG TCT TGA ACC AGT ATG TTT G
PVcre G1A forward (QM)CTA CAT ACA GTT CAA AAG CAA ACA CCG TAT TG
PVcre G1C forward (QM)CTA CAT ACA GTT CAA CAG CAA ACA CCG TAT TG
PVcre G1U forward (QM)CTA CAT ACA GTT CAA TAG CAA ACA CCG TAT TG
PVcre A14G forward (QM)CAA GAG CAA ACA CCG TGT TGA ACC AGT ATG TTT G
PVcre A14U forward (QM)CAA GAG CAA ACA CCG TTT TGA ACC AGT ATG TTT G
PV/Luc+(NheI) forw. (QM)GTT TTA TAC TCC CTT CCC GCT AGC TTA GAC GCA CAA AAC CAA G
PVcre (NheI) forwardCTA GCT AGC ACT ATT AAC AAC TAC
PVcre (NheI) reverseCTA GCT AGC TAC TAG CAA ACA TAC
Artificial cre (NheI) forwardCTA GCT AGC GAG CTC GAG CAA ACA CCG TAG AGC
Artificial cre (NheI) reverseGAT GCT AGC GAG CTC TAC GGT GTT TGC TCG AGC
Artificial cre A5C(NheI) forw.CTA GCT AGC GAG CTC GAG CCA ACA CCG TAG AGC
Artificial cre A5C(NheI) rev.GAT GCT AGC GAG CTC TAC GGT GTT GGC TCG AGC
PVcre (SLtm) forward (QM)CTA TTA ACA ACT ACA TAC AAT TTA AGA GCC AAC ACC GTA TCG AAC
  • a QM, oligonucleotide used for QuikChange mutagenesis.