TABLE 1.

Statistics of the cryoEM reconstructions

SampleNo. of micrographsIncubation time (min)Defocusa (μm)MagnificationPixel size (Å)No. of particles (no. selected/total no.)Correlation coefficientbResolutionc (Å)
PV1-glycoCD15524151.6-4.245,0002.912,022/4,7990.32815
PV1-deglycoCD15515151.5-3.445,0002.881,109/2,4040.32616
PV2-glycoCD15513151.2-4.645,0002.93512/1,1590.30821
PV2-deglycoCD15515151.7-4.145,0002.90613/1,3090.32421
PV3-glycoCD15510152.0-4.045,0002.91303/9310.40823
PV3-deglycoCD15510152.0-5.045,0002.88842/1,7590.35919
  • a Determined from the phase-contrast transfer function of the microscope.

  • b Mean real-space correlation coefficient (CC), calculated as follows: Embedded Image. In this formula, ρi, is the electron density of the boxed cryoEM image, ρm is the electron density of the model projection, and r is the radius of the corresponding density point used to assure proper weighting of the densities. The angle brackets indicate mean values.

  • c Resolution at which the correlation between two independent three-dimensional reconstructions falls below 0.5.