Sample | No. of micrographs | Incubation time (min) | Defocus^{a} (μm) | Magnification | Pixel size (Å) | No. of particles (no. selected/total no.) | Correlation coefficient^{b} | Resolution^{c} (Å) |
---|---|---|---|---|---|---|---|---|

PV1-glycoCD155 | 24 | 15 | 1.6-4.2 | 45,000 | 2.91 | 2,022/4,799 | 0.328 | 15 |

PV1-deglycoCD155 | 15 | 15 | 1.5-3.4 | 45,000 | 2.88 | 1,109/2,404 | 0.326 | 16 |

PV2-glycoCD155 | 13 | 15 | 1.2-4.6 | 45,000 | 2.93 | 512/1,159 | 0.308 | 21 |

PV2-deglycoCD155 | 15 | 15 | 1.7-4.1 | 45,000 | 2.90 | 613/1,309 | 0.324 | 21 |

PV3-glycoCD155 | 10 | 15 | 2.0-4.0 | 45,000 | 2.91 | 303/931 | 0.408 | 23 |

PV3-deglycoCD155 | 10 | 15 | 2.0-5.0 | 45,000 | 2.88 | 842/1,759 | 0.359 | 19 |

↵

*a*Determined from the phase-contrast transfer function of the microscope.↵

*b*Mean real-space correlation coefficient (CC), calculated as follows: . In this formula, ρ, is the electron density of the boxed cryoEM image, ρ_{i}is the electron density of the model projection, and_{m}*r*is the radius of the corresponding density point used to assure proper weighting of the densities. The angle brackets indicate mean values.↵

*c*Resolution at which the correlation between two independent three-dimensional reconstructions falls below 0.5.