TABLE 1.

Listing of all TAV ORFs that are assumed to code for viral proteinsa

TAV ORFNucleotide positionsLength (aa)Potential site(s) and signature(s) (amino acid position within protein sequence)Most-homologous protein(s) (accession no.); possible function% I/% S
003R (E1A)451-828126pRB-binding motif (62-66), zinc finger motif (95-115)HadV-B E1A protein (X03000); exon 1, transcription activator21.8/27.9
007R (E1A)896-100336HadV-B E1A protein (X03000); exon 2, transcription activator21.8/27.9
008R (E1BS)1008-1418137E1BS_ADEB3 (AF030154); small T antigen22.2/40.1
009R (E1BL)1361-2533391E1BL_ADEB3 (NC001876); large T antigen, transformation-associated protein31.4/38.0
011R (IX)2562-2975138Leucine zipper pattern (84-105), putative DNA gyrase/topoisomerase subunit A (90-124)HEX9_ADEP5 (NP108656); virion morphogenesis-associated protein32.6/33.3
014L (IVa2)2978-4333452NTP-binding site (173-180)PIV2_ADE17 (NP597866); transcriptional activator62.2/26.5
016L (pol)4078-6978967Conserved region I (777-782), two zinc finger motifs (3-27, 823-857)DPOL_ADECC (NP044189); DNA pol65.4/24.2
018R5240-547980Y115_ADE07 (P03288)51.2/14.9
023R7034-732196
024L (pTP)7332-8996555Sequence for protein-primed DNA replication (57-63), arginine-rich region (264-300), bipartite NLS (290-300), glutamic acid-rich region (304-349), NSGD motif (481-484)TERM_ADEP5 (NP108659); terminal protein64.2/24.2
028R (p52K)9154-10209352Putative hydroxylmethylglutaryl-coenzyme A reductase motif (83-89)L52_ADE17 (NC001460)62.0/24.9
031R (pIIIa)10237-11856540HEX3_ADE17 (NC002067); peripentonal hexon-associated protein56.8/28.3
033R (III)11923-13350476Fiber-interacting domain (234-245)PEN3_ADEP3 (AAC99444); penton base protein73.1/19.0
037R (pVII)13359-13751131Protease cleavage site (20-24), alanine-rich region (50-102), arginine-rich region (72-130), bipartite NLS (96-112)VCO7_ADECU (NC001734); major core protein37.5/36.8
040R (V)13821-14912364Three bipartite NLSs (69-85, 314-330, 319-335), arginine-rich region (315-335)VCOM_ADE02 (P03267); minor core protein43.1/25.8
045R (pX)14943-1514668Arginine-rich region (2-34), two protease cleavage sites (25-29, 35-40), two bipartite NLSs (18-35, 20-37)L2MU_ADECC (Q65953); core protein62.0/19.7
047R (pVI)15202-15942247Proline-rich region (175-214), two endoprotease cleavage sites (30-37, 233-240)PIV6_ADEP5 (NP108666); hexon-associated protein47.0/31.6
050R (hexon)15997-18729911Glycine-rich region (118-142), glucosaminoglycane attachment site (381-384), yeast PIR protein repeat (404-421)HEX_ADEP5 (NP108667); hexon protein77.5/16.3
060R (protease)18735-19346204Peptidase C5 (1-133)ADEN_ADECU (P35990), protease66.2/25.1
062L (T-DBP)19390-20508373Two zinc binding motifs (129-200, 240-309)DNB2_ADEP5 (NP108669); DBP24.4/36.5
067L (DBP)20540-21940467Two bipartite NLSs (62-65, 103-107), two zinc binding motifs (228-299, 338-407)DNB2_ADEB3 (AAD09730); DBP48.5/33.5
075R (100K)21951-24005685Carboxyltransferase domain (580-607), glucosaminoglycane attachment site (484-487)L100_ADEP5 (NP108670); hexon assembly-associated protein58.3/28.5
078L23003-23326108YL13_ADE41 (P23690)25.7/13.3
080R (33K)23827-24237137Proline-rich region33K_ADEB10 (AAL86535); virion morphogenesis-associated protein25.2/19.9
082R (pVIII)24487-25167227Two protease cleavage sites (108-115, 153-160)HEX8_ADEP5 (NP108672); hexon-associated protein68.7/18.5
084R25133-25993287
086R25965-2615062
087R (E3 14.5)26143-26529129E314_ADE05 (P04493)28.0/38.6
088L (U exon)26537-2672563U exon BAdV3 (54)27.0/39.7
090R (fiber)26703-28595631NLS (2-4), penton-base-interacting sequence (8-15), repeat/shaft region (201-422), last repeat motif (434-442), TLWT motif (458-461), head domain (459-631)FIBP_ADEB2 (AF308811); fiber protein29.5/42.1
095L (E434)28824-29555244Bipartite NLS (193-209)E434_ADE40 (Q64865)33.9/32.7
096L29539-2980589
099L (E411k)29808-30149114E411_ADE05 (P04489); nuclear binding protein29.7/43.2
101L30149-30463105
102L30473-3076698
103L30760-31062101
104L (dut)31065-31511149DUT_RAT (P70583)51.5/17.2
DUT_ADEG1 (Q89662); DUT42.7/19.5
105R31938-33242435Prokaryotic membrane lipoprotein lipid attachment site (133-143)
  • a % I/% S, percentage of amino acids identical (I) and similar (S) to the corresponding homologous proteins; HadV-B, human adenovirus B; ADEB3 (BadV3), bovine adenovirus type 3; ADEP5, porcine adenovirus type 5; ADE17, human adenovirus type 17; ADECC, canine adenovirus type 1; ADE07, human adenovirus type 7; ADEP3, porcine adenovirus type 3; ADECU, canine adenovirus type 1; ADE02, human adenovirus type 2; ADE41, human adenovirus type 41; ADEB10, bovine adenovirus type 10; ADEB2, bovine adenovirus type 2; ADE40, human adenovirus type 40; ADE05, human adenovirus type 5; ADEG1, fowl adenovirus type 1; NTP, nucleoside triphosphate. The homology values of the TAV E1A exons 003R and 007R are given for the complete corresponding protein of human adenovirus B (HAdV-B). The individual ORFs are numbered in the order in which they appear in the TAV genome, with the designation L for theoretical transcription to the left or R for theoretical transcription to the right. Missing numbers, represent TAV ORFs that were declared not to code for viral proteins and were therefore omitted from the table. Out of a total of 109 ORFs, 38 ORFs were selected to code for viral proteins according to the criteria described in Materials and Methods. The names written in parentheses in the first column correspond to the names of the corresponding homologous proteins of other adenoviruses. The sites and signatures found in the amino acid sequences of the potential TAV proteins were determined by the program PROSITE of the PC/GENE software package and the ExPASy Molecular Biology Server and by homology searches to known proteins of other adenoviruses. These sites and signatures are putative, and future experiments have to confirm their actual functionality.