TABLE 1.

Oligonucleotide primers used to establish TREx BCBL1-Rta cells

PrimerSequence (5′ → 3′)Location
P1CAGCTGTGGAATGTGTGTCAGTTAGSV40 promoter
P2GTGCTGCAAGGCGATTAAGTTGGGlacZ-Zeocin
P3CGCAAATGGGCGGTAGGCGTGCMV promoter
P4GGACCCACTTTCACATTTAAGTTGTet Repressor
P5GTAGTGTATTGACCGATTCCTTGCHygromycin
P6ATGGCGCAAGATGACAAGGRta
P7GTCTCGGAAGTAATTACGCCATTGGRta
P8CAGTGATCTCCTTCTGCATCCTGTCβ-Actin
P9GCTACGTCGCCCTGGACTTCGAGβ-Actin