TABLE 2.

B1 PCR of transduced mouse muscle

B1 PCR templatesB1 PCR results
Mouse quadricepsaWk after injectionNo. of vector copies/nucleusbFold amplification (vector)cNo. of repeatsd
B46351.1 ± 0.14
C16540.9 ± 0.14
D36221.1 ± 0.34
E26181.0 ± 0.24
C212131.0 ± 0.24
E31551.4 ± 0.24
E51581.5 ± 0.34
G12462.1 ± 0.74
G22441.8 ± 0.14
H42452.2 ± 0.84
J13221.1 ± 0.24
J23221.8 ± 0.54
I45721.5 ± 0.54
  • a Transduced muscles were harvested from individual mice at the indicated time after injection. Total cellular DNA was isolated from the entire muscle, and 20 to 40 ng were used in each reaction.

  • b Number of rAAV genomes per nucleus in the given sample as assayed by TaqMan PCR. A total of 1.5 × 104 input vector copies were assayed by B1 PCR for each mouse.

  • c Fold amplification, number of copies amplified divided by the number of input copies (see Fig. 1). Vector, rAAV genome analyzed.

  • d Number of experimental replicates.