TABLE 4.

Activities and sensitivities to compound 1 of recombinant proteasesa

EnzymeStrainMutation(s)SubstrateCompound 1
Km (μM)Kcat (min−1)IC50 (μM)Ki (μM)
BKBKC428S4290.012
Con1Con1E176GC428S4320.0100.006
D168YpCon1D168YE176GC428S835108.5
D168VpCon1D168VE176GC428S716178.5
  • a Data are averages of at least two separate experiments.