TABLE 1.

Accumulation of BWYV P3 mutants in agroinoculated plantsa

MutantMean absorbance in plantsb
Expt 1Expt 2Expt 3Expt 4
R7A/R8A0.01 ± 0.01
S70A0.90 ± 0.101.30 ± 0.14
T83A0.61 ± 0.140.94 ± 0.18
Q121E/N122D0.51 ± 0.111.36 ± 0.25
W166R0.00 ± 0.01
D168N0.42 ± 0.120.83 ± 0.25
E171Q0.85 ± 0.111.15 ± 0.14
D172N0.16 ± 0.030.18 ± 0.06
N137D/D172N0.40 ± 0.13
BW0 0.80 ± 0.090.77 ± 0.061.73 ± 0.150.75 ± 0.13
  • a Plant extracts were tested for BWYV by DAS-ELISA about 4 weeks after agroinfection with the indicated construct.

  • b Mean absorbance ± standard error at 405 nm after 1 h of substrate incubation. The background absorbance observed with healthy plant extract (typically 0.11 to 0.12) has been substracted. Each independent experiment was performed with 10 to 23 plants per construct.