TABLE 1.

Effects of sCD4-17b against genetically diverse HIV-1 isolates

ExptaIsolateCladebPhenotype (coreceptor usage)b,c% Neutralization by sCD4-17b at:
6.4 nM32 nM
1Ba-LBNSI (R5)9498
LAVBSI (X4)5075
292RW023ANSI (R5)00
Ba-LBNSI (R5)2699
91US054Bns00
92US714BNSI (R5)080
92US076BSI (R5X4)1184
93IN905CNSI (R5)76
92UG024DSI (X4)065
93TH073ENSI (R5)00
93BR029FNSI (R5)140
  • a In experiment 1, viruses were preincubated with the indicated concentrations of sCD4-17b in medium and assayed for infectivity, by using MAGI-CCR5 cells for Ba-L and MAGI cells for LAV. The numbers of blue cells per well in untreated controls were 350 for Ba-L and 300 for LAV. In experiment 2, viruses were preincubated with the indicated concentrations of affinity chromatography-enriched sCD4-17b and assayed for infectivity by using MAGI-CCR5 cells. For the different viruses, the numbers of blue cells per well in the untreated controls ranged from 50 to 475.

  • b Clade and coreceptor usage phenotypes are as specified in the AIDS Research and Reference Reagent Program catalogue.

  • c ns, not specified.