TABLE 1.

Summary of diagnostic testing by antigen-capture ELISA and RT-PCR during the EHF outbreak in Gulu, Uganda

AssayNo. of samples testedNo. of positive samplesNo. of false positivesaNo. of false negativesbNo. of PCR+ Ag+ samplesNo. of PCR+ Ag samples
Early detectionLate detection
Antigen-capture ELISA1,77128210
RT-PCR1,083246 3e4
Total19617c20d
  • a False positives were defined as samples that initially tested positive by a particular assay but tested negative with a subsequent sample from the same patient and for which all other diagnostic tests were and continued to be negative.

  • b False negatives were defined as samples that tested negative by nested RT-PCR and positive by antigen capture.

  • c Represents 13 patients for which the maximum time between PCR+ Ag samples was 72 to 96 h.

  • d Represents 18 patients for which the maximum time between PCR+ Ag samples was 48 to 72 h.

  • e There were an additional 10 potential false positives that were unresolvable.