TABLE 1.

Primer and probe sequences used for real-time PCR

GeneORF 73 (68 bp)aORF 50 (73 bp)a
Forward primer5′-CGCGAATACCGCTATGTACTCA-3′5′-CGCAATGCGTTACGTTGTTG-3′
Reverse primer5-GGAACGCGCCTCATACGA-3′5′-GCCCGGACTGTTGAATCG-3′
Taqman probe6FAM-ACATCACCACCCCACAGACCTGGAG-TAMRA6FAM-ACCTGTGCCCCCTCTTCGACACC-TAMRA
  • a Product length.