TABLE 2.

Primer sequences used for RT-PCR

GeneForward primerReverse primerProduct size (bp)
ORF75′-ACTGTGGGGGTCTGTCATCT-3′5′-GGTTGAAGTTGGGCTCTATC-3′377
ORF85′-CTGTATCCGCCAAGTTCG-3′5′-GGTCAAACACGCTACTCG-3′432
ORF95′-AGGAAAAGAGCAGTCAGGTC-3′5′-CGTAGGGGATTCTGTCGTGT-3′324
ORFK25′-AAAACACGCACCGCTTGACCTG-3′5′-TTCACTGCTGGTATCTGGAACG-3′534
ORFK45′-GCATCCTGCTCGTCGCTGTG-3′5′-GCTTCTTCACCCAGTCTTTC-3′240
ORFK55′-GCAGAGAAAACCGAGCAC-3′5′-GGGAAGAGGTGGGGAACG-3′332
ORFK65′-GCTGCCTAACCCAGTTTTTG-3′5′-TTACCTGACGCCCACAGAAA-3′209
ORF255′-GTCCACCCCTTCTTTGATTTTT-3′5′-TTTCCCGAGTTGACCCAGTAGG-3′335
ORF505′-GCCCTCTGCCTTTTGGTT-3′5′-GATGATGCTGACGGTGTG-3′351
ORFK85′-ACTGACGGTGGGGAAAAC-3′5′-GCGTGGGAGAATGTGACT-3′535
ORFK135′-ATTGACATTAGGGCATCC-3′5′-AAAGGAGGAGGGCAGGTT-3′327
ORF725′-GATAATAGAGGCGGGCAATG-3′5′-TAAAGCAGGTGTCCAAAGAA-3′338
ORF735′-GAAGTGGATTACCCTGTTGTTAGC-3′5′-TTGGATCTCGTCTTCCATCC-3′307
vIRF25′-CGGAATGGCTCACGGACTTTAT-3′5′-AGACATCCTTCACATCCCTTGT-3′346