TABLE 1.

Analysis of SIVmac239 variants with substitutions of isoleucine 324a

aa exchangeMean coreceptor usage ± SDReplicationbGroup
R5mneGPR15husMagirhPBMC221-89CEMx174C8166PM-1
I100100100++++++++++wt
K0.5 ± 0.12.0 ± 0.51.4 ± 0.2I
R0.8 ± 0.30.6 ± 0.31.2 ± 0.9+, ddI
S0.4 ± 0.11.7 ± 0.21.1 ± 0.2I
V149.5 ± 53.483.4 ± 23.5134.2 ± 38.5++++++, d++, d++II
C9.9 ± 1.54.2 ± 0.33.1 ± 0.2+, d++, dd++, dND+, dIII
E4.1 ± 0.85.7 ± 1.17.6 ± 1.4(+)++ND+, dIII
Y1.4 ± 0.60.9 ± 0.11.7 ± 0.7++, dc+, d++, ddIII
H2.2 ± 0.35.2 ± 1.22.2 ± 1.0++ND++, dIV
N1.2 ± 0.14.6 ± 0.53.7 ± 0.7++ND+, dIV
P1.7 ± 0.214.7 ± 0.84.1 ± 0.5+++, dND+, dIV
W1.3 ± 0.15.5 ± 0.94.2 ± 0.7++, dND+, dIV
F49.5 ± 5.427.6 ± 1.7193.3 ± 47.7++, dd++, d++, dd++, dd+, ddV
L19.1 ± 2.617.1 ± 1.7260.3 ± 6.9++, d++, dd(+), ddV
M11.8 ± 1.222.1 ± 2.6384.2 ± 40.5++, d++, dND+, dV
T33.1 ± 3.69.5 ± 1.9113.4 ± 12.4++, dd++(+), dd++V
  • a The results of a representative experiment are shown; comparable results were obtained in an independent experiment. aa, amino acid. Coreceptor usage and replication are shown relative to 239wt activity.

  • b Peak reverse transcriptase activity: 60 to 100%; ++; 30 to 60%, +; 10 to 30%, (+), <10%, −; d, peak in reverse transcriptase activity 3 to 6 days delayed; dd, peak in reverse transcriptase activity more than 6 days delayed; ND, not done.

  • c Result obtained with PBMC from one of four donor animals tested.