TABLE 1.

Oligonucleotides used for PU.1 binding studiesa

OligonucleotideSequence (5′→3′)
5′PU.1ATA CTG TAG TTC CTC AAT ATA G
5′PU.1 C′TTC ACT ATA TTG AGG AAC TAC A
MidPU.1TCA ATA TAG TTC CGC ATT TGT G
MidPU.1 C′ACG TCA CAA ATG CGG AAC TAT A
3′PU.1CGT GTT AAG TTC CTG TTT TTA C
3′PU.1 C′TAC TGT AAA AAC AGG AAC TTA A
5′ + mid PU.1TCC TGT AGT TCC TCA ATA TAG TTC CGC A
5′ + mid PU.1 C′CAA ATG CGG AAC TAT ATT GAG GAA CTA CAG G
Mid + 3′ PU.1TCA ATA TAG TTC CGC ATT TGC TAC GCG TTA AGT TCC TG
Mid + 3′ PU.1 C′ATA CTG TAA AAA CAG GAA CTT AAC GCG TAG CAA ATG CGG AAC
  • a Double-stranded oligonucleotides were made by annealing positive-strand oligonucleotides to their complementary (C′) partners. The core of each PU.1 binding motif is underlined. Other known transcription factor binding motifs (Oct and CRE) were eliminated from the oligonucleotides by point mutations.