TABLE 3.

Hydrolysis of ISAV receptors on erythrocytes by recombinant SDAV 4-O-acetylesterasea

ErythrocytesTreatmentHemagglutination units/ml% Reduction
RabbitPBS20,480
Esterase
    3 h10,24050
    20 h2,56088
HorsePBS5,120
Esterase
    3 h2,56050
    20 h1,28075
  • a Rabbit and horse erythrocytes were treated with recombinant SDAV esterase for the times indicated, washed, adjusted to a concentration of 0.75%, and subjected to hemagglutinin titration with purified ISAV. As a control, cells were incubated with PBS.