TABLE 2.

Percentage of H6LT-expressing cells during viral latency and reactivationa

Transcript% of positive cells (copy no. of transcript in 1 H6LT positive cell) at indicated no. of days poststimulation
0357
Type I H6LTs4.3 ± 1.7 (79 ± 48)27.3 ± 13.2 (228 ± 53)20.7 ± 12.4 (191 ± 142)1.9 ± 1.4 (43 ± 7.5)
Type II H6LTs5.2 ± 1.0 (65 ± 11)11.0 ± 4.6 (131 ± 94)8.8 ± 3.7 (104 ± 75)1.6 ± 0.48 (34 ± 17)
Productive IE1 and IE2NDND0.07 ± 0.066.5 ± 6.1 (159 ± 118)
  • a Percentages of H6LT-positive cells during viral reactivation were estimated by the Reed-Muench method (29). The copy numbers of each type of transcript in one H6LT-positive cell are shown in the parentheses. The percentage of cells that showed the productive pattern is also shown. The average of four independent cultures ± the standard deviation is shown for each latent transcript. Expression of the H6LTs was confirmed by the double-nested RT-PCR method described previously (20) (data not shown). Day 0, cells not stimulated with TPA. ND, not detected.