TABLE 1.

Total number of epitope-specific CD8+ T cells per spleen at different time points after infection with MCMV or influenza virus or after immunization with peptide GP33a

Time postinfection (days)Total no. of epitope-specific CD8+ T cells/spleen (104) ± SD with epitope:
IE1/pp89 (PFU)NP366GP33
MCMV (106)MCMV (103)MCMV-NPInfluenza virusMCMV-GPGP33+CFA
8-1156.8 ± 202.4 ± 0.32.5 ± 1.155.8 ± 28.51.1 ± 0.525.1 ± 9.7
18-2231.3 ± 8.72.9 ± 0.82.9 ± 1.620.3 ± 102.0 ± 0.7
35-4526.5 ± 14.47.9 ± 2.86.9 ± 3.74.8 ± 0.910.4 ± 3.718.7 ± 6.4
100-16059 ± 13.124.5 ± 6.912.7 ± 4.85.2 ± 2.215.5 ± 6.310.9 ± 2.6
250-350115 ± 2845.8 ± 229.2 ± 5.33.9 ± 0.5
  • a Mice were infected or immunized with the indicated virus or antigen. At different time points thereafter the total numbers of epitope-specific CD8+ T cells per spleen were measured by fluorescence-activated cell sorting by using tetramer staining (IE1/pp 89 and NP366) or ICS (GP33). Mean numbers ± the SD are given for 4 to 12 mice per group. Data from one to three experiments with the same antigen or virus were pooled for calculation. Values of 106 and 103 indicate the virus dose in PFU used for infection.