TABLE 1.

Recovery of rHPIV1 bearing codon substitution mutations at amino acid position 942 in the L protein

VirusCodonaNo. of nucleotide changes needed to restore wild-type amino acidrHPIV1 mutant recoveredAdventitious coding mutations in L ORF
rHPIV1 wild typeTAT, TAC0+NDb
rHPIV1-Y942HCAC1+None
rHPIV1-Y942CcTGC1+None
rHPIV1-Y942FTTT1+None
rHPIV1-Y942NcAAC1+None
rHPIV1-Y942DcGAC1+None
rHPIV1-Y942SAGC2+None
rHPIV1-Y942WTGG2+None
rHPIV1-Y942QCAG2+S1302N
rHPIV1-Y942KAAA2dND
rHPIV1-Y942IATC2ND
rHPIV1-Y942EGAG2ND
rHPIV1-Y942McATG3+L1367S
rHPIV1-Y942AGCG3+None
rHPIV1-Y942TcACA3+None
rHPIV1-Y942GGGG3+None
rHPIV1-Y942VcGTG3+G1755Q
rHPIV1-Y942LcCTG3+None
rHPIV1-Y942RCGG3ND
rHPIV1-Y942PCCG3ND
  • a In the case of wild type, the two possible codons yielding the wild-type amino acid for tyrosine (Y) are shown; for each mutant, the codon chosen for introduction into rHPIV1 is shown.

  • b ND, not done. The L gene sequence was not confirmed either because the virus is the previously sequenced wild type or because the virus was not recovered.

  • c Virus was recovered with pTML containing the indicated codon substitution. The other viruses were recovered using wild-type pTML.

  • d −, not recovered.