TABLE 4.

Induction of ISG transcripts by IFN-α, IFN-γ, and poly(I)-poly(C)a

CellbTreatmentFold changec
STAT1αSTAT1βPKRISG12IRF-1IP10
Huh7IFN-α41.138.820.33123.110.0
RepIFN-α18.624.118.72,3743.3111.5
Huh7IFN-γ80.870.63.480.047.9198.8
RepIFN-γ31.333.54.574.345.5123.1
Huh7Poly(I)-poly(C)1.3−1.31.01.6−1.2−1.6
RepPoly(I)-poly(C)−1.11.1−1.52.2−1.11.3
CHIFNα2.03.63.89.31.412.6
CHPoly(I)-poly(C)3.78.95.340.42.2549.0
  • a Huh7 and clone 45 cell lines were treated with IFN-α (1,000 U/ml), polyIC (100 μg/ml), or IFN-γ (1,000 U/ml). Primary chimpanzee hepatocytes were treated with IFN-α (100 U/ml) or polyIC (50 μg/ml).

  • b Rep, replicon clone 45 cell line; CH, chimpanzee hepatocytes.

  • c Transcripts for various ISGs were quantified using TaqMan RT-PCR assays. The data are averages from duplicate cultures and are expressed as the fold change compared to the values for untreated cultures. The data for IFN-α and IFN-γ treatment are from the 24-h harvest, since this time point had the highest values. For polyIC, similar values were obtained for the 24- and 48-h time points. The 48-h values are shown to demonstrate that a delayed response was not missed. The chimpanzee hepatocytes were harvested at 72 h for both treatments. Additional cells were not available to repeat the chimpanzee hepatocyte study at multiple time points. The level of the replicon RNA was decreased by 104- and 31.2-fold in IFN-α- and IFN-γ-treated cells at the 48-h time point. No decrease in replicon RNA was observed following polyIC treatment.