TABLE 1.

Replication of chimeric bovine-human PIV3s at permissive and restrictive temperaturesa

VirusMean titer (log10TCID50/ml) at 32°CMean log10 reduction in virus titer at the indicated temperature (°C)
353637383940
BPIV3 Kansas8.70.00.10.20.81.11.3
rHPIV3 wt8.60.3−0.20.10.30.40.5
rHPIV3-NB8.5b0.50.50.4
rHPIV3-PB7.81.01.21.3
rHPIV3-MB8.00.41.52.1
rHPIV3-FBHNB8.20.50.60.7
rHPIV3-LB8.00.00.41.53.74.64.1
rHPIV3-LB(T1711I)7.01.61.44.2≥5.3≥5.6≥5.7
rHPIV3 cp457.80.91.32.23.0≥6.4≥6.4
  • a Data are the means of results of two or more titrations. Values in bold type are at or above the shutoff temperature, which is defined as a 100-fold or greater reduction in titer compared to the titer at 32°C after adjusting for the reduction in replication of the rHPIV3 wild-type control virus. rHPIV3cp45 is included as a well-characterized ts control virus.

  • b —, Data not gathered.