TABLE 6.

Titration of antiviral effect of ribavirina

Ribavirin concn (μM)Replicon (106 ge/μg of cell RNA)bFold reductioncGAPDH Ctd
05.8017.4
503.41.717.6
1002.52.317.6
2000.319.317.6
4000.0022,90017.6
  • a Clone 8 cells were plated and allowed to reach confluency for 3 days and then were treated with ribavirin for 9 days.

  • b Replicon RNA was quantified by TaqMan RT-PCR and expressed as copy number (ge) per microgram of cell RNA.

  • c The data are expressed as the fold change compared to the values for untreated cultures.

  • d The TaqMan RT-PCR assay for replicon RNA was multiplexed for the GAPDH mRNA to demonstrate a lack of overt toxicity or decrease in cellular mRNA in ribavirin-treated cells. GAPDH values are expressed as Ct, the amplification cycle at which the values exceeded the background threshold.