TABLE 2.

Colony-forming efficiency of replicon constructsa

Replicon RNAColony-forming efficiency (colonies/μg of replicon RNA)
Rep1bNeo≤5
Rep1bNeo-2204800
Rep1bNeo-14310
Rep1bNeo-1431/220480,000
Rep1aNeo0
Rep1b/aNeo0
Rep1b/aNeo-22040
Rep1b/aNeo-1431/22040
  • a Huh7 cells were transfected with various replicons, and the colony-forming efficiency in the presence of G418 was determined. Replicons were transfected at 10 μg/100-mm-diameter dish, except for Rep1bNeo-1431/2204, which was transfected at 10 ng of replicon RNA mixed with 10 μg of Huh7 RNA as carrier. Each replicon was tested in multiple experiments. Rep1bNeo frequently failed to yield colonies, but in a single experiment yielded 50 colonies/10 μg of transfected RNA, and these colonies were the source of the cell lines in Table 1.