Table 1.

Nucleotide substitutions between CK-adapted Beau-CKa and the Vero-adapted Beau-USb laboratory strain of IBV

GeneNo. of mutationsPosition (nt)Nucleotide changeCodon changeAmino acid change
S gene820421T→CGCT→GCCNone
20480G→AAGT→AATSer→Asn
20731T→ATTA→ATALeu→Ile
21403T→ATCA→ACASer→Thr
21711G→AGTG→GTANone
22252G→AGGG→AGGGly→Arg
22415A→CAAT→ACTAsn→Thr
22612T→ATTG→ATGLeu→Met
Gene 3
 ORF 3a123906T→CTGT→TGCNone
 ORF 3b0
 ORF 3c (E)124501C→TCAA→TAAGln→Stop
M gene224635G→CAGT→ACTSer→Thr
24746C→TGCA→GTAAla→Val
Noncoding125227C→TNAd NA
Gene 5
 ORF 5a125513G→TAGA→ATAArg→Ile
 ORF 5b0
N genec 0
  • a The sequence of Beau-CK was determined previously (6).

  • b The sequence, derived from Vero-adapted Beau-US, has been deposited under accession no. AJ311362.

  • c Beau-R contains the A27087→G substitution not present in Beau-CK or Beau-US.

  • d NA, not applicable.