Table 1.

Listing of potentially expressed ORFs in WSBV

ORFProduct position (length [aaa])Best matchb[source]BlastP scoreIdentity (%)Length (aa)Predicted structure and/or functionc(position)dPoly(A) signalc
WSV001300501–445 (1,684) L23982 , collagen type VII [Homo sapiens]930421,336Collagen, TM+
WSV0021118–495 (208)Nucleocapsid protein VP24, TM, SP+
WSV0041511–1200 (104)+∗∗
WSV0062425–1541 (295) U07025 , chitinase [Janthinobacterium lividum]635453Glycosyl hydrolase, Pro-rich cluster (147–175), TM SP
WSV0081749–2360 (204)Ser/Gly-rich region (4–80), TM+
WSV0092672–2388 (95)+
WSV0113051–6953 (1,301) AF128951 , flagellin [Escherichia coli]4118496TM, SP,+
WSV0133955–3716 (80)+
WSV0206604–6254 (117)
WSV0217645–7046 (200)TM
WSV0227250–7432 (61)+
WSV0238502–7645 (286)
WSV0259248–8556 (231)Ser/Glu-rich region (36–114) and basic region (130–200), TM
WSV02613936–9332 (1,535) AE003491 , snogene product [Drosophila melanogaster]7125185Acidic region (1406–1445), TM+
WSV03516983–14068 (972) X77514 , pupal cuticule protein [Galleria mellonella]4626111TM, SP
WSV03717000–20839 (1,280)Glu-rich cluster (578–636)+
WSV04520784–23726 (981)Acidic region (284–360), ATP/GTP binding motif, TM
WSV04721688–22047 (120)Basic region (24–59)+
WSV04922759–22145 (205)TM, SP
WSV05123710–24297 (196)+∗∗
WSV05324906–24664 (81)+
WSV05525153–24965 (63)SP+
WSV05625878–25201 (226)Cys2/His2-type zinc finger+
WSV05926631–27254 (208)+
WSV06329077–28334 (248) AF078683 , Ring-H2 finger protein RHA1a [Arabidopsis thaliana]444052Cys2/Cys2-type zinc finger+
WSV06430861–29080 (594)TM+
WSV06731092–31958 (289)NP-001062, thymidylate synthetase [Homo sapiens]39267287Thymidylate synthase
WSV06932125–32796 (224)Cys2/His2-type zinc finger+∗∗
WSV07332948–34213 (422)TM, SP+
WSV07634218–35045 (276)
WSV07735074–35964 (297)TM+
WSV07837245–36052 (398) AE003485 , CG11122 gene product [Drosophila melanogaster]4526238+
WSV07938917–37385 (511) AL022223 , putative protein [Arabidopsis thaliana]433369EF-hand calcium-binding motif; Ring finger protein-like; Ser/Asp-rich region (204–330)+
WSV08340718–38976 (581) AC018363 , putative protein kinase [Arabidopsis thaliana]4227102Protein kinase, Ser/Thr protein kinase active-site signature, TM+
WSV09142054–45488 (1,145)Glu/Ser-rich region (626–737), TM+
WSV09745175–45471 (99)+
WSV10045951–47822 (624) AC024128 , putative CBP [Arabidopsis thaliana]474160CBP; Cys2/Cys2-type zinc finger, TM+
WSV10748635–48943 (103)TM, SP+
WSV10850300–49083 (406)NP-012284, cell surface flocculin [Saccharomyces cerevisiae]5524237Membrane-associated protein, TM+
WSV11251809–50427 (461) Q89662 , dUTP pyrophosphatase [fowl adenovirus type 1]9037161dUTPase
WSV11552007–54910 (968)TM
WSV11955055–58186 (1,044)Acidic region (122–174)+∗∗
WSV12858948–60057 (370)Repeat region (23–325)+
WSV12958956–60026 (357) X73481 , mst101(2) [Drosophila hydei]8030302Repeat region (20–322)+
WSV13060581–60132 (150)+∗∗
WSV13162127–60676 (484)+
WSV13362204–63016 (271)TM
WSV13462991–63656 (222)+
WSV13663666–64049 (128)TM, SP+
WSV13765042–64014 (337)TM+∗∗
WSV13968659–65036 (1,208)
WSV14269118–68708 (137)
WSV14369265–76203 (2,313) AB037755 , KIAA1334 protein [Homo sapiens]4818554Repeat region (325–427); Asn-rich cluster (990–1094), TM+∗∗
WSV14675119–74922 (66)+
WSV14777653–76277 (459)+
WSV15078365–77451 (305)
WSV15179065–83372 (1,436)TM+
WSV16185707–83431 (759)Glu-rich cluster (97–134), Asn/pro-rich region (467–637)+
WSV16688980–85765 (1,072) AE003593 , CG10523 gene product [Drosophila melanogaster]493469Cys2/Cys2-type zinc finger, TM+
WSV17291607–89064 (848) P21524 , ribonucleoside-diphosphate reductase large chain [Saccharomyces cerevisiae]72848790Ribonucleotide reductase large subunit, TM+
WSV17792964–92647 (106)+
WSV17893229–94134 (302) T29757 , protein UNC-89 [Caenorhabditis elegans]4622216Repeat region (82–302), TM, SP+∗∗
WSV18194624–95739 (372)
WSV18495744–97366 (541)Cys2/Cys2-type zinc finger, TM+
WSV18897548–98786 (413) AF117061 , ribonucleotide reductase R2 subunit [Aedes albopictus]36459313Ribonucleotide reductase small subunit, TM+
WSV19198854–99786 (311) AJ133437 , deoxyribonuclease I [Penaeus japonicus]5432149Nuclease, TM, SP+
WSV192102885–99829 (1,019)TM+
WSV195103071–103841 (257)TM, SP+
WSV198103844–104677 (278)
WSV199104760–107327 (856) AF156271 , Ring finger protein terf [Homo sapiens]433072Ring-H2 finger motif, TM+
WSV206108550–109161 (204)+
WSV207109261–110085 (275)Proline rich, TM+∗∗
WSV209114953–110136 (1,606)TM+
WSV214115053–115292 (80) L41834 , nuclear protein [Ensis minor]594673DNA-binding protein+∗∗
WSV216118987–115406 (1,194)Protein-splicing signature, TM+
WSV220119057–121078 (674)+
WSV222121100–123631 (844) AK016037 , putative [Mus musculus]443258Ring-H2 finger motif, ATP/GTP binding motif, TM+∗∗
WSV226123758–126547 (930)TM+
WSV230126755–127000 (82)+∗∗
WSV231129006–127162 (615)TM+
WSV234130290–129409 (294)+
WSV235129611–129811 (67)+
WSV236130076–130306 (77)TM+
WSV237130566–131441 (292)
WSV238131481–132938 (486)3830139Gly-rich cluster (50–138), TM, SP+
WSV242132994–133893 (300)TM
WSV244133969–136341 (791)TM+∗∗
WSV249137589–139937 (783) AC024760 , contains similarity to TR [Caenorhabditis elegans]7227202Ring-H2 finger motif, repeat region (454–633)+
WSV252140111–141613 (501)+∗∗
WSV254141696–142538 (281)+
WSV256142545–143696 (384)TM, SP+
WSV259143760–144686 (309)+∗∗
WSV260147517–144752 (922)Asp/Glu/Ser-rich region (344–485), TM+∗∗
WSV267148612–147770 (281)+
WSV269150145–148679 (489)TM+
WSV270150675–150166 (170)+
WSV271150688–154341 (1,218) S59310 , probable membrane protein YMR317w [Saccharomyces cerevisiae]4620369Lys/Ser-rich region (455–526), TM
WSV277154557–156929 (791) D86346 , crystal protein [Bacillus thuringiensis]4123249TM+
WSV282159352–161253 (634)Ser-rich cluster (13–129), SP+
WSV284161263–161562 (100)TM, SP
WSV285161718–165017 (1,100) AE003824 , CG13185 gene product [Drosophila melanogaster]4825183ATP/GTP binding motif, TM+∗∗
WSV289169814–165120 (1,565)NP-011856, serine/threonine protein kinase [Saccharomyces cerevisiae]4625245Protein kinase, TM, SP+
WSV291167278–167532 (85)TM, SP+
WSV294170113–170730 (206)
WSV295170832–171458 (209)TM+∗∗
WSV299172439–171513 (309)TM, SP+∗∗
WSV302173075–172509 (189)
WSV303173178–175850 (891)NP-069209, transcription initiation factor IID [Archaeoglobus fulgidus]4423140TBP Cys2/Cys2-type zinc finger, TM+
WSV306175840–177096 (419)TM+
WSV308177124–178521 (466)+
WSV310178530–179345 (272)TM+
WSV311180036–179425 (204)Nucleocapsid protein VP26, TM, SP+∗∗
WSV313183817–180279 (1,180)Glu-rich region (37–358) and Pro-rich cluster (462–492), TM+
WSV321184132–184482(117)TM, SP
WSV322184499–185179(227)TM
WSV323185082–184819(88)+∗∗
WSV324185434–185189(82)+
WSV325185433–186827(465)TM, SP+
WSV327190743–188176(856)TM+∗∗
WSV331190094–190306(71)+
WSV332190876–193233(786)+∗∗
WSV333191135–190932(68)TM, SP+
WSV338194629–193331(433)TM, SP+∗∗
WSV339195503–194655(283)
WSV340196292–195510(261)
WSV342196697–196398(100)+
WSV343209342–196803(4,180)TM+∗∗
WSV344197221–197517(99)TM+
WSV349199510–199779(90)TM, SP+
WSV360209616–227846(6,077) U96166 , srpA [Streptococcus cristatus]4623222Leucine-zipper motif, TM+∗∗
WSV386228196–227993(68)TM, SP+
WSV387228375–230561(729)
WSV390230617–231579(321)+
WSV394231422–231724(101)+
WSV395231603–232796(398) T41553 , thymidylate kinase [Schizosaccharomyces pombe]15741200Thymidylate kinase; ATP/GTP binding motif+
WSV397232819–233331(171)
WSV398233383–233763(127)TM+
WSV399234330–233782(183)+
WSV403236679–238601(641)Ring-H2 finger motif, repeat region (435–494), SP+
WSV406238659–239435(259) T27927 , hypothetical protein ZK593.8 [Caenorhabditis elegans]5026175TM, SP+
WSV407240139–239459(227)+
WSV412240713–241189(159)+
WSV414241637–241275(121)Asp-rich cluster (63–86), TM, SP+∗∗
WSV415241775–243406(544)TM
WSV419243217–243795(193)
WSV421244242–244853(204)Envelope protein VP28, TM, SP+∗∗
WSV423247143–244954(730) T22255 , hypothetical protein F45H7.4 [Caenorhabditis elegans]4526165Protein kinase, TM+
WSV427249230–247362(623)EF-hand calcium-binding motif, TM
WSV432249151–249456(102)+
WSV433249426–253208(1,261)Pro-rich cluster (29–71), TM+
WSV440253297–255117(607)
WSV442255075–257474(800)ATP/GTP binding motif, TM+
WSV446257552–259129(526)ATP/GTP binding motif, TM, SP+
WSV447264975–259168(1,936) Z70204 , similarity to yeast hypothetical helicase [Caenorhabditis elegans]424052Helicase, ATP/GTP binding motif, Asp-protease motif, TM+
WSV455265079–265597(173)TM, SP
WSV457265606–266400(265)TM, SP+
WSV459266838–266446(131)TM+
WSV461267400–266930(157)
WSV462267399–267647(83)+
WSV464268584–267721(288)+
WSV465272423–268695(1,243)Cys2/Cys2-type zinc finger, TM+
WSV477274527–275150(208)Cys2/Cys2-type zinc finger, ATP/GTP binding motif+
WSV479276736–275210(509)Glu-rich cluster (467–485), TM+∗∗
WSV482277035–277571(179)TM+∗∗
WSV483277705–278076(124)+
WSV484278423–277776(216)TM+
WSV486278637–280973(779) AF154037 , surface protein PspC [Streptococcus pneumoniae]4624194Lysine-rich, TM
WSV489281865–281131(245)+
WSV492282176–282583(136)+
WSV493283360–282677(228)Acidic region (59–103)+∗∗
WSV495283754–284011(86)+
WSV497285773–284079(565)TM+∗∗
WSV500286706–286080(209)Cys2/Cys2-type zinc finger, ATP/GTP binding motif+
WSV502286606–289632(1,009) AL352992 , ariadne-like protein [Leishmania major]515133Cys2/His2, Cys2/Cys2-type zinc finger, ATP/GTP binding motif, TM, SP+
WSV508291298–289685(538)TM+∗∗
WSV513291720–292202(161)
WSV514292190–298774(2,195) X61920 , DNA polymerase III catalytic subunit [Saccharomyces cerevisiae]5224201DNA polymerase, TM+∗∗
WSV518293724–293275(150)SP+
WSV524298729–298526(68)+
WSV525299033–298821(71)TM, SP+
WSV526300432–299089(448)TM+
  • a aa, amino acid.

  • b Accession numbers are from the GenBank or SwissProt database.

  • c Function was deduced from the degree of amino acid similarity to either products of known genes or Prosite signatures. TM, transmembrane domains; SP, signal peptides.

  • d Positions of amino acid residues.

  • e +, polyadenylation signal present; −, signal absent. ∗, the transcription of the ORF was also verified by RT-PCR; ∗∗, the ORF was confirmed by cDNA sequencing.