Table 1.

Replication of gp41 N-glycan attachment site mutants

VirusReplication ina:
CEMx174221C8166-45RhPBMC
SHIV-KB9++++++++++++++++
g1+++++++ND++++
g2+++++++ND+++
g3+++++++ND+++
g4+++++++ND+++
g12+++ND+
g13+++++ND+++
g14+++++ND+++
g23+++++ND+++
g24++++++++++
g34+++++ND++
g123++++ND
g124+++++++
g134++++++++
g234+++++++ND
g1234ND
  • a +, replication was detectable by antigen capture assay; −, replication was below the limit of detection; ++++, peak replication similar to wild type; +++, slight delay (1 to 2 days) relative to wild type; ++, delayed (3 to 4 days) relative to wild type; ND, not done. The CEMx174, 221, and C8166-45 cell lines were tested by transfection; replication in rhesus monkey PBMC (RhPBMC) was tested by infection of 2 × 106 cells with viral supernatants normalized to contain 10 ng of p27 capsid antigen.