Table 2.

Relative copies for the panel of vectors within specific tissues as detected by Taqman PCRa

TissueRelative no. of copies (% of AdL value)b
AdLAdL.F*AdL.PB*AdL.PB*F*
Liver100 ± 2039 ± 1110 ± 3.47.4 ± 1.7
Spleen100 ± 4469 ± 1341 ± 16171 ± 36
Lung100 ± 24103 ± 537.4 ± 4.814 ± 4.5
Heart100 ± 2087 ± 309.3 ± 5.617 ± 6.6
Kidney100 ± 7722 ± 87.4 ± 5.24.8 ± 0.5
Diaphragm100 ± 4182 ± 7629 ± 188.8 ± 2.2
  • a Tissue DNA was analyzed using primers and probes that recognize the pIX region of Ad5. Results were obtained as the number of of copies per nanogram of DNA.

  • b To control for tissue differences, the results are reported as percentages of the number of copies of AdL detected in the respective tissue following subtraction of background. The values listed are expressed as mean ± standard error.