Table 2.

Comparison of preparations of recombinant C68 and human adenovirustype 5 virusesa

ConstructTotal yield (particles)Viral particle/PFU ratioRCA
C68-CMV-HIV-gagshort1 × 1014 31Negative
C68-CMV-Rab-gp7.5 × 1013 286Negative
C68-CMV-HIV-gagfull8 × 1013 178Negative
C68-CMVEGFP1.6 × 1014 570Negative
Mean ± SD
  C68 vectors (n = 4)1.04 (±0.39) × 1014 266 ± 228Negative
  Ad5 vectors (n = 53)4.5 (±4.2) × 1013 78 ± 8824% of prepns positive
  • a Total viral particle yield, ratio of viral particles to PFU, and results of RCA tests from four different C68 vector preparations and 53 preparations of human adenovirus type 5 (Ad5) vectors that contain different transgenes were compared. All preparations were from 50 plates (150 mm) of 293 infections. HIV-gag short and HIV-gag full represent different open reading frames of the gag gene of HIV. Rab-gp is the gene encoding rabies virus glycoprotein.