Table 2.

gp120 shedding

Cell typeTime (h) posttransfectionEnvelope protein in supernatant (%)a
Parentalg134g234g1234HxBC2
COS-74814.47.312.617.788.6
7214.822.722.059.389.6
9616.426.823.560.791.6
293T7222.938.734.868.794.3
  • a Values indicate gp120 present in cell-free supernatant as a percentage of total gp120 (supernatant plus whole-cell lysate). The HxBC2 construct used in this assay expressed a secreted form of gp120 protein.