Table 2.

Mapping the epitopes of anti-gp41 MAbs using synthetic peptides and recombinant proteins

AntigenAmino acid sequenceReactivity by ELISA
Z134E102F5
Peptides
 2030 (MN) QTQQEKNEQELLELDKWASL +
 2031 (MN)           LLELDKWASLWNWFDITNWLW +++
 2032 (MN)                         DITNWLWYIKI
 KGND                   KGWNWFDITNWGK-NH2 ++
 ND (JR-FL)                   Ac-NWFDITK-NH2 ±b ±
 SIVmac239 (671–690)             KLNSWDVFGNWFDLASWIKY
Recombinant proteins
 gp41(INT)IIIB        ...LLELDKWASLWNWFNITNWLV... ++
 gp41(VT)IIIB        ...LLELDKWASLWNWFNITNWLW... ++
 gp160(PS)IIIB        ...LLELDKWASLWNWFNITNWLW... ++
 gp140JR-FL, SOS-gp140JR-FL        ...LLELDKWASLWNWFDITNWLW...a +++
 gp14089.6        ...LLELDKWASLWNWFDITNWLW... +++
  • a Sequence sometimes appears with a K at the underlined position; the K variant was not tested.

  • b  ±, weak reactivity by ELISA.