Table 2.

Coreceptor usage by HIV-1 viruses isolated using CD8-depleted PBMC versus MDM as target cells

VirusTarget cells used for isolationCoreceptor usagea
ControlCCR2bCCR3CCR5CCR8CXCR4CX3CR1Gpr1Gpr15Strl33Apj
MACS1-brMDM+++
PBMC++++++
MACS1-spinMDM++++
PBMC+++++++
MACS3-brMDM+++
PBMC+++++/−
  • a The pattern of coreceptor usage of viruses isolated using CD8-depleted PBMC or MDM as target cells was determined in Cf2-Luc cells, as described for Fig. 3. Control cells were transfected with CD4 alone. Entry levels were scored as +++ (>50,000 luciferase activity units), ++ (between 30,000 and 50,000 luciferase activity units), + (between 10,000 and 30,000 luciferase activity units), +/− (between 5,000 and 10,000 luciferase activity units), or − (<5,000 luciferase activity units).