Table 1.

Experimental design for inoculation of Gn pigs with TC or WT Cowden PEC

Group no.Gn pigs (n)Inoculum (titer)aInoculation routeEuthanasia (PID)Age (days) at:
InoculationEuthanasia
I4TC PEC (106FFU/ml or 2,560 [by ELISA])Oral2–74–66–11
II9WT PEC (2,560 [by ELISA])Oral3–94–68–13
III1Serum-free EMEMOral44 8
IV3WT PEC (2,560 [by ELISA])i.v.6–84–610–12
V1Formalin-inactivated WT PECi.v.6612
VI2Serum-free EMEMi.v.8412
VII2WT PEC-positive acute serab (1:2 dilution, 100 [by ELISA])Oral or i.v.7–28411–32
VIII2WT PEC-positive acute serac (1:2 dilution, 100 [by ELISA])Oral or i.v.3–2147–25
  • a The TC PEC inoculum titer was determined by both CCIF and an antigen-ELISA. The WT PEC inoculum titer was determined by an antigen-ELISA. All pigs were given 10 to 14 ml of inoculum per pig.

  • b WT-PEC-positive acute sera were from Gn pigs (pigs 8–1 and 8–2) inoculated i.v. with WT PEC/Cowden and were determined as positive for PEC RNA and PEC antigens by RT-PCR and an antigen-ELISA, respectively.

  • c WT-PEC-positive acute sera were from Gn pigs (pigs 8–8 and 8–9) inoculated orally with WT PEC/Cowden and were determined as positive for PEC RNA and PEC antigens by RT-PCR and an antigen-ELISA, respectively.