Table 1.

Immortalization of transfected PBMCs by ACH-envelope clones

CloneaNo. of immortalized culturesb/no. of electroporation exptsp19 expression in immortalized cellsc
ACH.WTNTd NT
ACH-HTE3/5+
ACH-753/4+
ACH-812/5+
ACH-954/5+
ACH-1010/5
ACH-1054/5+
ACH-1954/5+
ACH-EN0/5
ACH-CT0/5
pBluescript KS0/5
ACH-101/VSV-G0/3
ACH-EN/VSV-G0/3
ACH-CT/VSV-G0/3
  • a Clones used for this assay are shown.

  • b Determined by MTT assays (31).

  • c Results of p19 antigen expression in immortalized cells at 90 days posttransfection, as previously described (33). + and − p19 expression of greater than 4 ng/ml and less than 25 pg/ml, respectively.

  • d NT, not tested.