Table 3.

Summary of diarrhea, fecal virus shedding, and virus in serum of Gn pigs after i.v. inoculation with WT PEC

Group no.aPig no.PID at euthanasiaClinical signs and virus sheddingDiarrheab or virus sheddingc at PID:
012345678
IVd 13–116Diarrhea+++
Fecal virus shedding−/−/NT+/−/NT+/−/NT+/+++/NT+/+++/NT+/+++/++/+++/+
Virus in serum−/−/NT+/+/NT+/−/NT+/+/NT
8–16Diarrhea++
Fecal virus shedding−/−/NT+/−/NT+/−/NT+/++/NT+/+/NT+/+++/NT+/+++/+
Virus in serum−/−/NT+/−/NT+/+/NT+/+/NT
8–28Diarrhea++++
Fecal virus shedding−/−/NT+/−/NT+/−/NT+/+/NT+/+++/NT+/+++/NT+/+++/NT+/+++/NT+/+++/+
Virus in serum−/−/NT−/+/NT+/+/NT+/+/NT+/+/NT
Ve 13–66Diarrhea
Fecal virus shedding−/−/NT−/−/NT−/−/NT−/−/NT−/−/NT−/−/NT−/−/−
Virus in serum−/−/NT−/−/NT−/−/NT−/−/NT
VIf 8–58Diarrhea
Fecal virus shedding−/−/NT−/−/NT−/−/NT−/−/NT−/−/NT−/−/NT−/−/NT−/−/NT−/−/−
Virus in serum−/−/NT−/−/NT−/−/NT−/−/NT−/−/NT
8–68Diarrhea
Fecal virus shedding−/−/NT−/−/NT−/−/NT−/−/NT−/−/NT−/−/NT−/−/NT−/−/NT−/−/−
Virus in serum−/−/NT−/−/NT−/−/NT−/−/NT−/−/NT
  • a All pigs were 4 to 6 days of age at inoculation.

  • b Symbols: +, diarrhea, fecal consistency of ≥2; ±, questionable diarrhea or fecal consistency of 1 to <2; −, no diarrhea or fecal consistency of 0 to <1.

  • c Fecal virus shedding determined by RT-PCR, ELISA, and IEM, respectively. Results shown are in this order, separated by slashes: (i) for RT-PCR and IEM, virus detected (+) or not detected (−); (ii) for ELISA, +++, A 492 > 1; ++, A 492 = 0.5 to 1;A 492 < 0.5, −, A492 <A + 3 SD; (iii) for IEM, NT, not tested.

  • d WT PEC.

  • e Killed WT PEC.

  • f Mock inoculation with serum-free EMEM.