Table 1.

Generation of recTHOV, following plasmid transfection of 293T cells

Hours after plasmid transfectionVirus titers in culture supernatant (PFU/ml) in expta:
1 (complete)2 (complete)3 (complete)4 (−T7/M)5 (−T7/M)
24  0 0 0 00
48 10 0 0 0 0
721.1 × 104 0  00 0
96 1 × 106 0 90 0 1
120 1.5 × 107 02.4 × 103 12.4 × 103
1447.5 × 107 0 4.4 × 104 3 × 103 8.7 × 104
172 5 × 107 0 1.5 × 106 4.6 × 104 2 × 107
  • a 293T cells were transfected with the full set of 12 expression plasmids (complete) or with all plasmids except the M-encoding helper plasmid (−T7/M). At different time points, the virus titer of the culture supernatants was determined.